alpha-Defensin 1 Antibody (2D2)

Western Blot: alpha-Defensin 1 Antibody (2D2) [NBP2-75406] - Analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody (2D2) [NBP2-75406] - Analysis of paraffin-embedded human spleen tissue using anti-HNP-1 antibody. Counter stained with hematoxylin.

Product Discontinued

alpha-Defensin 1 Antibody (2D2) Summary

• Immunogen: Synthetic peptide within Human alpha-Defensin 1 aa 1-50 / 94. (SwissProt: P59665 Human)
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DEFA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for alpha-Defensin 1 Antibody (2D2)

• alpha 2
• alphaDefensin 1
• alpha-Defensin 1
• DEF1
• DEF1DEFA1B
• DEFA1
• DEFA2
• defensin, alpha 1, myeloid-related sequence
• defensin, alpha 1HP-1
• HNP-1
• HNP-1HP1
• HP1
• MRS
• MRSMGC138393
• myeloid-related sequence
• neutrophil defensin 1

Background

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundantin the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine,respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequenceand distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8.The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likelyplays a role in phagocyte-mediated host defense. It differs from defensin, alpha 3 by only one amino acid. (providedby RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Defensin 1 Antibody (NBP2-75406)(1)

Publication using NBP2-75406

• Valle-Jimenez X, Ramirez-Cosmes A, Aquino-Dominguez AS, Sanchez-Pena F et al. Human platelets and megakaryocytes express defensin alpha 1 Platelets 2019-05-22 [PMID: 31116063]

Bioinformatics

• Gene Symbol: DEFA1