alpha-Aminoadipate Aminotransferase Antibody

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Western Blot: AADAT Antibody [NB110-99587] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

alpha-Aminoadipate Aminotransferase Antibody Summary

Immunogen
Peptide with sequence C-KPEDAKNPQKNTPK corresponding to internal region according to NP_057312.1, NP_872603.1.
Specificity
Both reported variants represent identical protein (NP_057312.1and NP_872603.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AADAT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 45 kDa band observed in human liver lysates (calculated MW of 47.4 kDa band according to NP_057312.1).
Control
alpha-Aminoadipate Aminotransferase Knockout 293T Cell Lysate
Publications
Read Publication using NB110-99587.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Aminoadipate Aminotransferase Antibody

  • AADAT
  • alphaAminoadipate Aminotransferase
  • alpha-Aminoadipate Aminotransferase
  • aminoadipate aminotransferase
  • EC 2.6.1.39,2-aminoadipate aminotransferase
  • EC 2.6.1.7,2-aminoadipate transaminase
  • KAT/AadAT
  • KAT2
  • KAT2mitochondrial
  • Kynurenine aminotransferase II
  • Kynurenine--oxoglutarate aminotransferase II
  • Kynurenine--oxoglutarate transaminase II
  • L kynurenine/alpha aminoadipate aminotransferase

Background

Aminoadipate aminotransferase (AADAT) is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Aminoadipate Aminotransferase Antibody (NB110-99587)(1)

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AADAT
Uniprot