alpha-2A Adrenergic R/ADRA2A Antibody

Immunocytochemistry/Immunofluorescence: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

alpha-2A Adrenergic R/ADRA2A Antibody Summary

• Description: Novus Biologicals Goat alpha-2A Adrenergic R/ADRA2A Antibody (NB100-2819) is a polyclonal antibody validated for use in ELISA, Flow and ICC/IF. Anti-alpha-2A Adrenergic R/ADRA2A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TERRPNGLGPERS corresponding to internal region according to NP_000672.2.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADRA2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
• Control:

  alpha-2A Adrenergic R/ADRA2A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for alpha-2A Adrenergic R/ADRA2A Antibody

• a-2A AdrenergicR
• ADRA2
• ADRA2A
• ADRA2Ralpha-2-adrenergic receptor, platelet type
• ADRARZNF32
• adrenergic, alpha-2A-, receptor
• Alpha-2 adrenergic receptor subtype C10
• alpha-2A Adrenergic R
• alpha-2A adrenergic receptor
• alpha2A AdrenergicR
• Alpha-2A adrenoceptor
• Alpha-2A adrenoreceptor
• alpha-2AAR subtype C10
• ALPHA2AAR
• Alpha-2AAR
• ZNF32

Background

This study investigates the involvement of alpha2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that alpha2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal alpha2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, alpha1D-AR, were not changed by the treatment. Moreover, alphao-subunits of GTP-binding proteins (Galphao), one of the downstream molecules of alpha2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of alpha2A-AR in the mouse hippocampus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-2A Adrenergic R/ADRA2A Antibody (NB100-2819)(2)

Publications using NB100-2819

• Shao F, Liu Z, Wei Q et al. FOXO1 orchestrates the intestinal homeostasis via neuronal signaling in group 3 innate lymphoid cells The Journal of experimental medicine 2023-10-02 [PMID: 37549024]
• Park L, Nigg JT, Waldman ID et al. Association and linkage of alpha-2A adrenergic receptor gene polymorphisms with childhood ADHD. Mol Psychiatry 2005-06-01 [PMID: 15520832]

Bioinformatics

• Gene Symbol: ADRA2A
• Entrez:
  • 150(Human)
  • 11551(Mouse)
• Uniprot:
  • NP_000672.2(Human)