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![Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot alpha-2A Adrenergic R/ADRA2A protein expression. Nitrergic neuron alpha2-,F(5, 12)=16.50,p<.0001 protein profiles are depicted in Panels 3B; GABAergic neuron alpha2-,F(5, 12)=10.47,p<.0001 protein profiles are presented in Panels 3E. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-2A Adrenergic R-ADRA2A Antibody-Western Blot-NBP2-22452-img0002.jpg "Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot alpha-2A Adrenergic R/ADRA2A protein expression. Nitrergic neuron alpha2-,F(5, 12)=16.50,p<.0001 protein profiles are depicted in Panels 3B; GABAergic neuron alpha2-,F(5, 12)=10.47,p<.0001 protein profiles are presented in Panels 3E. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.")
![Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), T47D (Lane 3) and HEL 92.1.7 (lane 4). The blots were probed with Anti-alpha-2a Adrenergic Receptor Rabbit Polyclonal Antibody.](http://images.novusbio.com/fullsize/alpha-2A-Adrenergic-R-ADRA2A-Antibody-Western-Blot-NBP2-22452-img0001.jpg)
![Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22452_rabbit-polyclonal-alpha-2a-adrenergic-r-adra2a-antibody-310202416113511.jpg)
![Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22452_rabbit-polyclonal-alpha-2a-adrenergic-r-adra2a-antibody-31020241613626.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/alpha-2A-Adrenergic-R-ADRA2A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2819-img0001.jpg "Immunocytochemistry/Immunofluorescence: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-2A-Adrenergic-R-ADRA2A-Antibody-Flow-Cytometry-NB100-2819-img0002.jpg)
![Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP3-10657] - Western blot analysis of alpha-2A Adrenergic R/ADRA2A in Fetal Thymus as a positive control. Antibody dilution at 1.0 ug/ml](http://images.novusbio.com/fullsize/alpha-2A-Adrenergic-R-ADRA2A-Antibody-Western-Blot-NBP3-10657-img0001.jpg "Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP3-10657] - Western blot analysis of alpha-2A Adrenergic R/ADRA2A in Fetal Thymus as a positive control. Antibody dilution at 1.0 ug/ml")
![Western Blot: alpha-2A Adrenergic R/ADRA2A Overexpression Lysate [NBL1-07357] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha 2a Adrenergic Receptor.](http://images.novusbio.com/fullsize/Alpha-2a-Adrenergic-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07357-img0002.jpg "Western Blot: alpha-2A Adrenergic R/ADRA2A Overexpression Lysate [NBL1-07357] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha 2a Adrenergic Receptor.")