alpha 1-Microglobulin Antibody (3F1) - Azide and BSA Free

Western Blot: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Analysis of AMBP expression in transfected 293T cell line by AMBP monoclonal antibody (M01), clone 3F1.Lane 1: AMBP transfected lysate(39 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Analysis of monoclonal antibody to AMBP on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Analysis of monoclonal antibody to AMBP on formalin-fixed paraffin-embedded human liver. Antibody concentration 1 ug/ml.

Western Blot: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - AMBP monoclonal antibody (M01), clone 3F1 Analysis of AMBP expression in A-549.

ELISA: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Detection limit for recombinant GST tagged AMBP is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

alpha 1-Microglobulin Antibody (3F1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AMBP (AAH41593, 19 a.a. ~ 352 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGPVPTPPDNIQVQGNFNISRIYGKWYNLAIGSTCPWLKKIMDRMTVSTLVLGEGATEAEISMTSTRWRKGVCEETSGAYEKTDTDGKFLYHKSKWNITMESYVVHTNYDEYAIFLTKKFSRHHGPTITAKLYGRAPQLRETLLQDFRVVAQGVGIPEDSIFTMADRGECVPGEQEPEPILIPRVRRAVLPQEEEGSGGGQLVTEVTKKEDSCQLGYSAGPCMGMTSRYFYNGTSMACETFQYGGCMGNGNNFVTEKECLQTCRTVAACNLPIVRGPCRAFIQLWAFDAVKGKCVLFPYGGCQGNGNKFYSEKECREYCGVPGDGDEELLRFSN
• Specificity: AMBP - alpha-1-microglobulin/bikunin precursor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AMBP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for alpha 1-Microglobulin Antibody (3F1) - Azide and BSA Free

• A1M
• alpha 1Microglobulin
• alpha 1-Microglobulin
• alpha-1-microglobulin/bikunin precursor
• AMBP
• bikunin
• EDC1
• HCP
• HCPcomplex-forming glycoprotein heterogeneous in charge
• HI30
• IATIL
• inter-alpha-trypsin inhibitor light chain
• ITI
• ITIL
• ITILC
• ITILgrowth-inhibiting protein 19
• protein AMBP
• protein HC
• trypstatin
• uristatin
• uronic-acid-rich protein
• UTI

Background

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 1-Microglobulin Antibody (H00000259-M01)(1)

Publication using H00000259-M01

• Cheng PJ, Wang TH, Huang SY et al. Differential proteomics analysis of amniotic fluid in pregnancies of increased nuchal translucency with normal karyotype. Prenat Diagn. 2011-02-10 [PMID: 21312199]

Bioinformatics

• Gene Symbol: AMBP
• Entrez:
  • 259(Human)
• OMIM:
  • 176870(Human)
• Uniprot:
  • AAH41593(Human)