Alix Antibody (JM85-31)

Western Blot: Alix Antibody (JM85-31) [NBP2-75403] - Western blot analysis of Alix on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate

Immunohistochemistry-Paraffin: Alix Antibody (JM85-31) [NBP2-75403] - Analysis of paraffin-embedded rat stomach tissue using anti-ALIX antibody. Counter stained with hematoxylin.

Flow Cytometry: Alix Antibody (JM85-31) [NBP2-75403] - Analysis of Jurkat cells with ALIX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Alix Antibody (JM85-31) [NBP2-75403] - Analysis of ALIX on different cell lysates using anti-ALIX antibody at 1/1,000 dilution.Positive control: Lane 1: Hela, Lane 2: PC-12, Lane 3: NIH-3T3

Immunohistochemistry-Paraffin: Alix Antibody (JM85-31) [NBP2-75403] - Analysis of paraffin-embedded human colon cancer tissue using anti-ALIX antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Alix Antibody (JM85-31) [NBP2-75403] - Analysis of paraffin-embedded human prostate tissue using anti-ALIX antibody. Counter stained with hematoxylin.

Product Discontinued

Alix Antibody (JM85-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Alix aa 1-160 / 868. (SwissProt: Q8WUM4 Human; SwissProt: Q9WU78 Mouse; SwissProt: Q9QZA2 Rat)
• Localization: Cell junction, Cytoplasm, Cytoskeleton, Secreted, Tight junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDCD6IP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Alix Antibody (JM85-31)

• AIP1DRIP4
• ALG-2 interacting protein 1
• ALG-2 interacting protein X
• ALG-2-interacting protein 1
• alinx
• Alix
• apoptosis-linked gene 2-interacting protein X
• dopamine receptor interacting protein 4
• HP95
• KIAA1375
• MGC17003
• PDCD6-interacting protein
• programmed cell death 6 interacting protein
• programmed cell death 6-interacting protein

Background

Alix encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD6IP