Alix Antibody (3C4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Alix Antibody (3C4) [H00010015-M01] - Analysis of monoclonal antibody to PDCD6IP on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: Alix Antibody (3C4) [H00010015-M01] - Detection limit for recombinant GST tagged PDCD6IP is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alix Antibody (3C4) - Azide and BSA Free Summary

• Immunogen: PDCD6IP (AAH20066, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVK
• Specificity: PDCD6IP - programmed cell death 6 interacting protein
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD6IP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Alix Antibody (3C4) - Azide and BSA Free

• AIP1DRIP4
• ALG-2 interacting protein 1
• ALG-2 interacting protein X
• ALG-2-interacting protein 1
• alinx
• Alix
• apoptosis-linked gene 2-interacting protein X
• dopamine receptor interacting protein 4
• HP95
• KIAA1375
• MGC17003
• PDCD6-interacting protein
• programmed cell death 6 interacting protein
• programmed cell death 6-interacting protein

Background

This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alix Antibody (H00010015-M01)(2)

Publications using H00010015-M01

• Y Ogawa, Y Akimoto, M Ikemoto, Y Goto, A Ishikawa, S Ohta, Y Takase, H Kawakami, M Tsujimoto, R Yanoshita Stability of human salivary extracellular vesicles containing dipeptidyl peptidase IV under simulated gastrointestinal tract conditions Biochemistry and Biophysics Reports, 2021-06-03;27(0):101034. 2021-06-03 [PMID: 34141904]
• Boonyaratanakornkit J, Schomacker H, Collins P, Schmidt A. Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system. PLoS One. 2013-01-01 [PMID: 23527201] (ICC/IF)

Bioinformatics

• Gene Symbol: PDCD6IP
• Entrez:
  • 10015(Human)
• OMIM:
  • 608074(Human)
• Uniprot:
  • AAH20066(Human)