ALG2 Overexpression Lysate (Native)

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Western Blot: ALG2 Overexpression Lysate (Adult Normal) [NBL1-07467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALG2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ALG2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204766 and based on accession number NM_033087. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae) (ALG2), transcript variant 1, mRNA.
Gene
ALG2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ALG2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ALG2 Overexpression Lysate (Native)

  • alpha-1,3-mannosyltransferase ALG2
  • alpha-1,3-mannosyltransferase)
  • asparagine-linked glycosylation 2 homolog (S. cerevisiae
  • asparagine-linked glycosylation 2 homolog (yeast
  • asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S.cerevisiae)
  • Asparagine-linked glycosylation protein 2 homolog
  • CDGIi
  • EC 2.4.1
  • EC 2.4.1.-
  • FLJ14511
  • GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
  • hALPG2
  • homolog of yeast ALG2
  • NET38

Background

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) represents the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ALG2 Lysate (NBL1-07467) (0)

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Bioinformatics

Gene Symbol ALG2