Aldo-keto Reductase 1C4/AKR1C4 Antibody

Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody [NB100-40786] - Analysis of Human Liver lysate (35ug protein in RIPA buffer). at 0.1ug/ml.

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody [NB100-40786] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody [NB100-40786] - (0.1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aldo-keto Reductase 1C4/AKR1C4 Antibody Summary

• Description: Novus Biologicals Goat Aldo-keto Reductase 1C4/AKR1C4 Antibody (NB100-40786) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence DPKYQRVELNDGH-C corresponding to N-Terminus according to NP_001809.2.
• Specificity: This antibody may cross-react with AKR1C1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AKR1C4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 37.1 kDa band according to NP_001809.2). IHC-P: Human liver shows staining of distinct structures in the cytoplasm of hepatocytes.
• Control:

  Aldo-keto Reductase 1C4/AKR1C4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aldo-keto Reductase 1C4/AKR1C4 Antibody

• 3-alpha-HSD
• AKR1C4
• Aldo-keto Reductase 1C4
• aldo-keto reductase family 1, member C4
• AldoketoReductase 1C4
• C11
• CDR
• CHDR
• DD4
• DD-4
• HAKRA

Background

The AKR1A1 gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C4
• Entrez:
  • 1109(Human)
• Uniprot:
  • NP_001809.2(Human)