ALDH7A1 Antibody - Azide and BSA Free

Western Blot: ALDH7A1 Antibody [NBP2-99843] - Anti-ALDH7A1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: SH-SY5Y Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa (We are unsure as to the identity of these extra bands).

Immunohistochemistry-Paraffin: ALDH7A1 Antibody [NBP2-99843] - Immunochemical staining of human ALDH7A1 in human liver (from 2 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections. The image showing positive staining of hepatocyte.

Immunohistochemistry-Paraffin: ALDH7A1 Antibody [NBP2-99843] - Immunochemical staining of human ALDH7A1 in human hepatoma (from 3 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: ALDH7A1 Antibody [NBP2-99843] - ALDH7A1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 ul anti-ALDH7A1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ALDH7A1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ALDH7A1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human ALDH7A1 (Uniprot#: P49419-2; Ser 2-Gln 511).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH7A1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunohistochemistry-Paraffin 1:5000-1:25000
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ALDH7A1 Antibody - Azide and BSA Free

• aldehyde dehydrogenase 7 family, member A1
• Aldehyde dehydrogenase family 7 member A1,26g turgor protein homolog
• Alpha-AASA dehydrogenase
• ATQ1Antiquitin-1
• Betaine aldehyde dehydrogenase
• Delta1-piperideine-6-carboxylate dehydrogenase
• EC 1.2.1
• EC 1.2.1.3
• EC 1.2.1.31
• EC 1.2.1.8
• EPDalpha-aminoadipic semialdehyde dehydrogenase
• FLJ11738
• FLJ92814
• P6c dehydrogenase
• PDEantiquitin-1

Background

Antiquitin (Aldh7a1) is an evolutionarily conserved protein believed to play a role in the regulation of cellular turgor. Based on sequence analysis, this protein is classified as a member of the aldehyde dehydrogenase superfamily (1). Analysis of the amount of mRNA in various rat and human tissues indicates that the largest amounts are found in rat kidney and liver and in cultured human hepatoma cells. Only minimal amounts were detected in human peripheral blood leukocytes, rat lung, or cultured human fibroblasts (2). The plant homolog of ATQ1 is thought to be involved in regulating turgor pressure, a function that also would be essential for cells of the mammalian cochlea. Northern blots of 13 human fetal tissues show antiquitin to be highly expressed in cochlea, ovary, eye, heart, and kidney (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH7A1