ALDH6A1 Antibody (6H9B7) - BSA Free

Western Blot: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Analysis using ALDH6A1 mAb against human ALDH6A1 (AA: 1-195) recombinant protein. (Expected MW is 47.2 kDa)

Immunocytochemistry/Immunofluorescence: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Analysis of Hela cells using ALDH6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Analysis of paraffin-embedded bladder cancer tissues using ALDH6A1 mouse mAb with DAB staining.

Flow Cytometry: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Analysis of Hela cells using ALDH6A1 mouse mAb (green) and negative control (red).

Western Blot: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Analysis using ALDH6A1 mAb against HEK293 (1) and ALDH6A1 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Analysis of paraffin-embedded ovarian cancer tissues using ALDH6A1 mouse mAb with DAB staining.

ELISA: ALDH6A1 Antibody (6H9B7) [NBP2-52411] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 6H9B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ALDH6A1 Antibody (6H9B7) - BSA Free Summary

• Description: Novus Biologicals Mouse ALDH6A1 Antibody (6H9B7) - BSA Free (NBP2-52411) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human ALDH6A1 (AA: 1-195) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH6A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 57.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ALDH6A1 Antibody (6H9B7) - BSA Free

• aldehyde dehydrogenase 6 family, member A1
• Aldehyde dehydrogenase family 6 member A1
• EC 1.2.1.18
• EC 1.2.1.27
• Malonate-semialdehyde dehydrogenase [acylating]
• malonate-semialdehyde dehydrogenase
• methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
• MGC40271
• MMSADHA
• MMSDHmitochondrial acylating methylmalonate-semialdehyde dehydrogenase

Background

ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH6A1