ALDH5A1 Antibody

Western Blot: ALDH5A1 Antibody [NBP1-52099] (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099] - (5g/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ALDH5A1 Antibody Summary

• Immunogen: Peptide with sequence C-SQDPAQIWRVAE corresponding to internal region according to NP_001071.1.
• Epitope: C-SQDPAQIWRVAE
• Specificity: This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALDH5A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 58.7 kDa band according to NP_733936.1). Please note that both this product and EB10436 detects a 50 kDa band in mouse and rat liver lysates (calculated MW of 56.0 kDa band according to mouse NP_766120.1). IHC-P: Human liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH5A1 Antibody

• aldehyde dehydrogenase 5 family, member A1
• EC 1.2.1
• EC 1.2.1.24
• NAD(+)-dependent succinic semialdehyde dehydrogenase
• SSADHmitochondrial

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH5A1 Antibody (NBP1-52099)(1)

Publication using NBP1-52099

• Joslyn G, Ravindranathan A, Brush G et al. Human variation in alcohol response is influenced by variation in neuronal signaling genes. Alcohol Clin Exp Res 2010-05-01 [PMID: 20201926]

Bioinformatics

• Gene Symbol: ALDH5A1
• Entrez:
  • 7915(Human)
  • 214579(Mouse)
  • 291133(Rat)
• Uniprot:
  • NP_733936.1(Human)