ALDH5A1 Antibody

Images

 
Western Blot: ALDH5A1 Antibody [NBP1-52099] (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099] - (5g/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

ALDH5A1 Antibody Summary

Immunogen
Peptide with sequence C-SQDPAQIWRVAE corresponding to internal region according to NP_001071.1.
Epitope
C-SQDPAQIWRVAE
Specificity
This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALDH5A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 58.7 kDa band according to NP_733936.1). Please note that both this product and EB10436 detects a 50 kDa band in mouse and rat liver lysates (calculated MW of 56.0 kDa band according to mouse NP_766120.1). IHC-P: Human liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.
Publications
Read Publication using NBP1-52099.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ALDH5A1 Antibody

  • aldehyde dehydrogenase 5 family, member A1
  • EC 1.2.1
  • EC 1.2.1.24
  • NAD(+)-dependent succinic semialdehyde dehydrogenase
  • SSADHmitochondrial

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALDH5A1 Antibody (NBP1-52099)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALDH5A1
Entrez
Uniprot