ALDH2 Antibody (OTI4H2)

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4H2) [NBP2-02102] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2.

Flow Cytometry: ALDH2 Antibody (4H2) [NBP2-02102] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH2 antibody, and then analyzed by flow cytometry.

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4H2) [NBP2-02102] - Immunofluorescent staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF
• Clone: OTI4H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ALDH2 Antibody (OTI4H2) Summary

• Immunogen: Full length human recombinant protein of human ALDH2 (NP_000681) produced in HEK293T cell.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
• Theoretical MW: 54.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH2 Antibody (OTI4H2)

• acetaldehyde dehydrogenase 2
• aldehyde dehydrogenase 2 family (mitochondrial)
• aldehyde dehydrogenase, mitochondrial
• ALDH class 2
• ALDH2
• ALDH-E2
• ALDHI
• ALDM
• EC 1.2.1
• EC 1.2.1.3
• liver mitochondrial ALDH
• MGC1806
• nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH2
• Entrez:
  • 217(Human)