ALDH2 Antibody (OTI4H2) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2.

Flow Cytometry: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ALDH2 antibody, and then analyzed by flow cytometry.

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151] - Staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF, CyTOF-ready
• Clone: OTI4H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ALDH2 Antibody (OTI4H2) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ALDH2 (NP_000681) produced in HEK293T cell.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
• Theoretical MW: 54.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ALDH2 Antibody (OTI4H2) - Azide and BSA Free

• acetaldehyde dehydrogenase 2
• aldehyde dehydrogenase 2 family (mitochondrial)
• aldehyde dehydrogenase, mitochondrial
• ALDH class 2
• ALDH2
• ALDH-E2
• ALDHI
• ALDM
• EC 1.2.1
• EC 1.2.1.3
• liver mitochondrial ALDH
• MGC1806
• nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH2 Antibody (NBP2-70151)(1)

Publication using NBP2-70151

• Wang Z, Shen J, Ye K et al. Neutrophil-Derived IL-6 Potentially Drives Ferroptosis Resistance in B Cells in Lupus Kidney Mediators of Inflammation 2023-05-27 [PMID: 37273451] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: ALDH2