ALDH1A3 Antibody (CL11968) - Azide and BSA Free

Analysis in human prostate and skeletal muscle tissues using NBP3-44356 antibody. Corresponding ALDH1A3 RNA-seq data are presented for the same tissues.

Staining of human anaplastic oligodendroglioma shows strong cytoplasmic positivity in a subset of cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human prostate cancer shows strong cytoplasmic positivity in tumor cells.

Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Analysis in human cell line U-2197.

Staining of PC-3 cells using the Anti-ALDH1A3 monoclonal antibody, showing specific staining in cytosol in green. Nuclear probe is visualized in blue.

Staining of human testis shows moderate to strong cytoplasmic positivity in peritubular myoid cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL11968
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ALDH1A3 Antibody (CL11968) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ALDH1A3 Antibody (CL11968) - Azide and BSA Free (NBP3-15126) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P47895, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ALDH1A3 Antibody (CL11968) - Azide and BSA Free

• aldehyde dehydrogenase 1 family, member A3
• Aldehyde Dehydrogenase 1-A3
• Aldehyde dehydrogenase 6
• aldehyde dehydrogenase family 1 member A3
• ALDH1A3
• ALDH1A6
• ALDH6
• ALDH6acetaldehyde dehydrogenase 6
• EC 1.2.1
• EC 1.2.1.5
• MCOP8
• RALDH3
• RALDH-3
• Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A3