Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate (Native)

Images

 
Western Blot: ALDH3A1 Overexpression Lysate (Adult Normal) [NBL1-07455] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202440 and based on accession number NM_000691. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 2, mRNA.
Gene
ALDH3A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Aldehyde Dehydrogenase 3-A1/ALDH3A1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate (Native)

  • aldehyde dehydrogenase 3 family, member A1
  • Aldehyde dehydrogenase 3
  • Aldehyde Dehydrogenase 3A1
  • Aldehyde Dehydrogenase 3-A1
  • Aldehyde dehydrogenase family 3 member A1
  • aldehyde dehydrogenase isozyme 3
  • aldehyde dehydrogenase type III
  • ALDH3A1
  • ALDH3aldehyde dehydrogenase, dimeric NADP-preferring
  • ALDHIII
  • EC 1.2.1
  • EC 1.2.1.5
  • MGC10406
  • stomach aldehyde dehydrogenase

Background

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ALDH3A1
Entrez