Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - (0.01ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - Staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody Summary

• Immunogen: Peptide with sequence C-RYPPSPAKMTQH corresponding to C-Terminus according to NP_000682.3.
• Epitope: C-RYPPSPAKMTQH
• Specificity: Reported variants represent identical protein: NP_001128639.1, NP_000682.3, NP_001128640.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALDH3A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3-5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in mouse eye lysates (calculated MW of 50.5 kDa band according to mouse NP_031462.2). IHC-P: Human Lung shows strong staining of the respiratory epithelium of the tracheal wall.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

• aldehyde dehydrogenase 3 family, member A1
• Aldehyde dehydrogenase 3
• Aldehyde Dehydrogenase 3A1
• Aldehyde Dehydrogenase 3-A1
• Aldehyde dehydrogenase family 3 member A1
• aldehyde dehydrogenase isozyme 3
• aldehyde dehydrogenase type III
• ALDH3A1
• ALDH3aldehyde dehydrogenase, dimeric NADP-preferring
• ALDHIII
• EC 1.2.1
• EC 1.2.1.5
• MGC10406
• stomach aldehyde dehydrogenase

Background

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (NBP1-52052)(1)

Publication using NBP1-52052

• Bogucka M, Giebultowicz J, Zawada K et al. The oxidation status of ALDH3A1 in human saliva and its correlation with antioxidant capacity measured by ORAC method. Acta Pol Pharm 2009-09-01 [PMID: 19894643]

Bioinformatics

• Gene Symbol: ALDH3A1
• Entrez:
  • 218(Human)
• Uniprot:
  • NP_000682.3(Human)