Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [Alexa Fluor® 350]

• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Aldehyde Dehydrogenase 3‑A1/ALDH3A1
  Ser2-His453
  Accession # P30838
• Specificity: Detects human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ALDH3A1 (Aldehyde dehydrogenase 3; also aldehyde dehydrogenase, dimeric NADP-preferring) is a 51-55 kDa, nuclear and cytosolic member of the ALDH3 family, ALDH superfamily of enzymes. It has widespread expression, being found in pancreatic acinar cells, corneal epithelium and stromal keratocytes, stem cells, and likely the epithelium of the jejunum. ALDH3A1 has many activities, including the detoxification of lipid peroxidation-derived medium chain (>5 carbon) aldehydes, the absorption of UV light in the cornea, acting as an antioxidant through the production of NADPH, and the extension of the cell cycle in expressing cells by reducing cyclin-dependent kinase activity. Notably, the critically important absorption of UVB light by ALDH3A1 in the cornea induces its aggregation and renders the molecule inactive as an enzyme. Human ALDH3A1 is 453 amino acids (aa) in length. It contains four acetylated lysines and a general catalytic region between aa 5-447. There are potentially six splice variants, one of which shows a 130 aa substitution for aa 1-247. Over aa 2-453, human ALDH3A1 shares 80% and 61% aa sequence identity with mouse ALDH3A1 asnd human ALDH3A2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.