Akt2 Overexpression Lysate (Native)


Western Blot: AKT2 Overexpression Lysate (Adult Normal) [NBL1-07442] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT2.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Akt2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC217733 and based on accession number NM_001626. The protein contains a C-terminal DDK tag.
C-terminal Myc/DDK
Homo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.6 kDa

Lysate Details for Akt2

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Akt2 Overexpression Lysate (Native)

  • EC 2.7.11
  • EC
  • Murine thymoma viral (v-akt) homolog-2
  • PKB beta
  • PKBB
  • Protein kinase Akt-2
  • Protein kinase B beta
  • rac protein kinase beta
  • RAC-beta serine/threonine-protein kinase
  • RAC-beta
  • RAC-PK-beta
  • v-akt murine thymoma viral oncogene homolog 2


This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: IHC
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Simple Western

Publications for Akt2 Lysate (NBL1-07442) (0)

There are no publications for Akt2 Lysate (NBL1-07442).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Akt2 Lysate (NBL1-07442) (0)

There are no reviews for Akt2 Lysate (NBL1-07442). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Akt2 Lysate (NBL1-07442) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Akt2 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for Akt2 Lysate (NBL1-07442)

Discover related pathways, diseases and genes to Akt2 Lysate (NBL1-07442). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Akt2 Lysate (NBL1-07442)

Discover more about diseases related to Akt2 Lysate (NBL1-07442).

Pathways for Akt2 Lysate (NBL1-07442)

View related products by pathway.

PTMs for Akt2 Lysate (NBL1-07442)

Learn more about PTMs related to Akt2 Lysate (NBL1-07442).

Research Areas for Akt2 Lysate (NBL1-07442)

Find related products by research area.

Blogs on Akt2.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

The Akt Antibody Plays Many Roles in Cancer Research
There are three human isoforms of the AKT gene, which plays a key role in several signalling pathways. Akt antibody studies have shown the Atk kinases to play a diverse number of roles within the cell, regulating angiogenesis, apoptosis, protein synth...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol AKT2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-07442 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought