Recombinant Human AKR7A3 His Protein

SDS-Page: Recombinant Human AKR7A3 Protein [NBP1-40394] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Human AKR7A3 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-331 of Human AKR7A3
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR

• Details of Functionality: Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AKR7A3
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 41.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human AKR7A3 His Protein

• AFAR2
• AFB1 aldehyde reductase 2
• AFB1-AR 2
• aflatoxin B1 aldehyde reductase 2
• aflatoxin B1 aldehyde reductase member 3
• aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)

Background

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AKR7A3