Airway Trypsin-like Protease/HAT/TMPRSS11D Antibody (337029)

• Reactivity: Hu
• Applications: WB
• Clone: 337029
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Airway Trypsin-like Protease/HAT/TMPRSS11D Antibody (337029) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Airway Trypsin-like Protease/HAT
  Ala42-Ile418
  Accession # O60235
• Specificity: Detects human Airway Trypsin-like Protease/HAT in direct ELISAs and Western blots. Recognizes the catalytic-domain of recombinant human HAT.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMPRSS11D
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Airway Trypsin-like Protease/HAT/TMPRSS11D Antibody (337029)

• airway trypsin like protease
• Airway trypsin-like protease
• EC 3.4.21
• EC 3.4.21.-
• HAT
• MGC150587
• MGC150588
• TMPRSS11D
• transmembrane protease serine 11D
• transmembrane protease, serine 11D

Background

Human Airway Trypsin‑like protease (HAT), also known as Transmembrane Protease, Serine 11D (TMPRSSIID), is a type II transmembrane serine protease that is expressed in the cells of the submucosal serous glands of the bronchi and trachea (1‑3). The deduced sequence predicts a short cytoplasmic tail (aa 120), a transmembrane domain (aa 21‑41), and an ectodomain consisting of a SEA domain (aa 44‑164) and a catalytic domain (aa 187‑418). The single‑chain precursor can be converted into a disulfide‑linked two‑chains, one of which corresponds to the catalytic domain. HAT was initially purified from the sputum of patients with chronic airway diseases (1). HAT has been shown to induce PAR‑2 mediated IL‑8 release in psoriasis vulgaris and increase mucin expression in airway epithelial cells (4, 5). The amino acid sequence of human HAT is 99%, 79%, and 67% identical to that of chimpanzee, canine and rat/mouse.

1. Yasuoka, S. et al. (1997) Am. J. Respir. Cell Mol. Biol. 16:300.
2. Yamaoka, K. et al. (1998) J. Biol. Chem. 273:11895.
3. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
4. Iwakiri, K. et al. (2004) J. Invest. Dermatol. 122:937.
5. Chokki, M. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:470.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Airway Trypsin-like Protease/HAT/TMPRSS11D Antibody (MAB2695)(1)

Publication using MAB2695

• Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts. PLoS ONE, 2012;7(4):e35876. 2012 [PMID: 22558251] (IHC-P, Human)

Bioinformatics

• Gene Symbol: TMPRSS11D
• Uniprot:
  • O60235()