Human Agrin ELISA Kit (Chemiluminescence)

ELISA: Human Agrin ELISA Kit (Chemiluminescence) [NBP2-66661] - Samples were spiked with high concentrations of Human Agrin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Agrin ELISA Kit (Chemiluminescence) [NBP2-66661] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human Agrin ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human AGRN in samples. No significant cross-reactivity or interference between Human AGRN and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.28%
• Intra-Assay: CV% < 10.7%
• Spike Recovery: 88-10%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: AGRN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Agrin ELISA Kit (Chemiluminescence)

• agrin proteoglycan
• Agrin
• AGRN

Background

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AGRN