AGAP3 Antibody

Immunohistochemistry-Paraffin: AGAP3 Antibody [NBP1-40081] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

AGAP3 Antibody Summary

• Immunogen: Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:32000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA 1:32000
• Application Notes: IHC: NBP1-40081 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AGAP3 Antibody

• ANK repeat and PH domain-containing protein 3
• ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
• centaurin-gamma-3
• cnt-g3
• CRAG
• CRAM-associated GTPase
• CRMP (collapsin response mediator protein) associated
• FLJ16146
• FLJ34452
• gamma 3
• MR1-interacting protein
• MRIP-1

Background

CENTG3, or M-RIP, is a 116 kDa protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGAP3