AFAP1L2 Antibody

Images

 
Western Blot: AFAP1L2 Antibody [NBP1-77358] - Mouse liver tissue lysate with AFAP1L2 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: AFAP1L2 Antibody [NBP1-77358] - Immunofluorescence of AFAP1L2 in human liver tissue with AFAP1L antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: AFAP1L2 Antibody [NBP1-77358] - Human liver tissue with AFAP1L2 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AFAP1L2 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AFAP1L2. The immunogen is located within the last 50 amino acids of AFAP1L2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AFAP1L2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
AFAP1L2 Peptide (NBP1-77358PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AFAP1L2 Antibody

  • actin filament associated protein 1-like 2
  • actin filament-associated protein 1-like 2
  • AFAP1-like protein 2
  • FLJ14564
  • KIAA1914Em:AC005383.4
  • XB130CTB-1144G6.4

Background

AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF482
Species: Mu
Applications: WB, IHC
NB200-118
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-33404
Species: Hu
Applications: IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-33581
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-20680
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15971
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-46403
Species: Hu
Applications: IHC
NBP1-89077
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-83642
Species: Hu
Applications: IHC, IHC-P
NBP2-46498
Species: Hu
Applications: WB, IHC
NBP1-89942
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-45745
Species: Hu
Applications: WB, IHC, IF
NBP2-13980
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77358
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for AFAP1L2 Antibody (NBP1-77358) (0)

There are no publications for AFAP1L2 Antibody (NBP1-77358).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AFAP1L2 Antibody (NBP1-77358) (0)

There are no reviews for AFAP1L2 Antibody (NBP1-77358). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AFAP1L2 Antibody (NBP1-77358) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional AFAP1L2 Products

Bioinformatics Tool for AFAP1L2 Antibody (NBP1-77358)

Discover related pathways, diseases and genes to AFAP1L2 Antibody (NBP1-77358). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AFAP1L2 Antibody (NBP1-77358)

Discover more about diseases related to AFAP1L2 Antibody (NBP1-77358).
 

Pathways for AFAP1L2 Antibody (NBP1-77358)

View related products by pathway.

PTMs for AFAP1L2 Antibody (NBP1-77358)

Learn more about PTMs related to AFAP1L2 Antibody (NBP1-77358).

Blogs on AFAP1L2

There are no specific blogs for AFAP1L2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AFAP1L2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol AFAP1L2
Uniprot