Afamin Antibody

Western Blot: Afamin Antibody [NBP1-51973] - Analysis of AFM in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-51973 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Afamin Antibody Summary

• Description: Novus Biologicals Goat Afamin Antibody (NBP1-51973) is a polyclonal antibody validated for use in WB and ELISA. Anti-Afamin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NKDDRPKDLSLRE corresponding to internal region according to NP_001124.1.
• Epitope: C-NKDDRPKDLSLRE
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AFM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in human brain (cerebellum and cerebral cortex) lysates (calculated MW of 69.1 kDa band according to NP_001124.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Afamin Antibody

• Afamin
• AFM
• ALB2
• ALB2alpha-Alb
• ALBA
• ALBAalpha-albumin
• ALF
• Alpha-Alb
• Alpha-albumin
• MGC125338
• MGC125339

Background

This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Afamin Antibody (NBP1-51973)(1)

Publication using NBP1-51973

• Kratzer I, Bernhart E, Wintersperger A et al. Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier. J Neurochem 2009-02-01 [PMID: 19046407]

Bioinformatics

• Gene Symbol: AFM
• Entrez:
  • 173(Human)
  • 75547(Mouse)
  • 293024(Rat)
• Uniprot:
  • NP_001124.1(Human)