Adenosine A1R Antibody

Product Discontinued

Adenosine A1R Antibody Summary

• Immunogen: Peptide with sequence NDHFRCQPAPPIDED corresponding to C-Terminus according to NP_001041695.1.
• Specificity: Both reported isoforms represent identical protein (NP_000665.1; NP_001041695.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADORA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave bands at approx. 30 kDa band and 20 kDa band in human brain (cerebellum) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 36.5 kDa band according to NP_000665.1 and NP_001041695.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Adenosine A1R Antibody

• Adenosine A1 R
• adenosine A1 receptor
• Adenosine A1R
• ADORA 1
• ADORA1
• RDC7
• RDC7adenosine receptor A1

Background

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenosine A1R Antibody (NBP1-00149)(1)

Publication using NBP1-00149

• Funakoshi H, Chan TO, Good JC et al. Regulated overexpression of the A1-adenosine receptor in mice results in adverse but reversible changes in cardiac morphology and function. Circulation 2006-11-21 [PMID: 17088462]

Bioinformatics

• Gene Symbol: ADORA1
• Entrez:
  • 134(Human)
  • 11539(Mouse)
  • 29290(Rat)
• Uniprot:
  • P30542(Human)