Adenosine A1R Antibody (1051024) [Unconjugated]

Adenosine A1R was detected in immersion fixed paraffin-embedded sections of liver cancer using Mouse Anti-Human Adenosine A1R Monoclonal Antibody (Catalog # MAB11135) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1051024
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Adenosine A1R Antibody (1051024) [Unconjugated] Summary

• Immunogen: Human Adora-1 synthetic peptide corresponding to the 2nd. extra-cellular loop.
  
  Accession # P30542
• Specificity: Detects human Adora-1 in direct and cell-based elisas
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adenosine A1R Antibody (1051024) [Unconjugated]

• Adenosine A1 R
• adenosine A1 receptor
• Adenosine A1R
• ADORA 1
• ADORA1
• RDC7
• RDC7adenosine receptor A1

Background

Adenosine A1 Receptor (ADORA1, Adenosine A1R) is an adenosine receptor that belongs to the G protein-coupled receptor family (GPCR). There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The Adenosine A1R receptor inhibits adenylyl cyclase, and plays a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P30542(Human)