ADAMTS15 Antibody

Western blot shows lysates of HEK293 human embryonic kidney cell line, BG01V human embryonic stem cells, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse ADAMTS15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human/Mouse ADAMTS15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5149) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

ADAMTS15 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS15
  Gly18-Cys682
  Accession # Q8TE58
• Specificity: Detects human and mouse ADAMTS15 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human ADAMTS1, L1.2, L2, 3, 4, 5, 8, 10, 12, 13, and 16 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ADAMTS15
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS15 Antibody

• A disintegrin and metalloproteinase with thrombospondin motifs 15
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein
• ADAM metallopeptidase with thrombospondin type 1 motif, 15
• ADAM-TS 15
• ADAMTS15
• ADAM-TS15
• ADAMTS-15
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82
• metalloprotease disintegrin 15 with thrombospondin domains
• MGC126403

Background

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) is a group of nineteen secreted zinc binding metalloproteases that is involved in development, inflammation, angiogenesis and several pathological conditions (1, 2). They are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. The mature secreted enzymes consist of catalytic, disintegrin-like, type I thrombospondin (TSP) motif, cysteine-rich, and spacer domains followed by zero to fourteen additional TSP motifs. Among ADAMTS proteases, ADAMTS1, -4, -5, -8, and -15 form a subfamily that possess aggrecanase activity (1 - 3). While ADAMTS4 and -5 (also known as aggrecanse-1 and -2, respectively) (4) have been well studied, the enzymatic activity of ADAMTS15 remains to be explored. It is suggested that ADAMTS15 may be involved in aggrecan degradation and breast cancer (5, 6). ADAMTS15 may also exhibit tumor suppressor activities since it contains genetic mutations in human colorectal carcinomas and restrains tumor growth and invasion in both in vitro and in vivo studies (7). The purified rhADAMTS15 is truncated and ends before the spacer domain.

1. Porter, S. et al. (2005) Biochem. J. 386:15.
2. Jones, G.C. and G.P. Riley (2005) Arthritis Res. Ther. 7:160.
3. Nicholson, A.C. et al. (2005) BMC Evol. Biol. 5:11.
4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
5. Porter, S. et al. (2006) Intl. J. Cancer 118:1241.
6. Kevorkian, L. et al. (2004) Arthritis Rheum. 50:131.
7. Viloria, C.G. et al. (2009) Cancer Res. 69:4926.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAMTS15
• Uniprot:
  • Q8TE58()