Human ADAMTS18 ELISA Kit (Colorimetric)

ELISA: Human ADAMTS18 ELISA Kit (Colorimetric) [NBP2-66442] - Samples were spiked with high concentrations of Human ADAMTS18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human ADAMTS18 ELISA Kit (Colorimetric) [NBP2-66442] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human ADAMTS18 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ADAMTS18 in samples. No significant cross-reactivity or interference between Human ADAMTS18 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.77%
• Intra-Assay: CV% < 4.7%
• Spike Recovery: 84-111%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ADAMTS18

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ADAMTS18 ELISA Kit (Colorimetric)

• A disintegrin and metalloproteinase with thrombospondin motifs 18
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 18
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 21
• ADAM metallopeptidase with thrombospondin type 1 motif, 18
• ADAM-TS 18
• ADAM-TS18
• ADAMTS-18
• ADAMTS21
• disintegrin and metalloprotease-like protein
• EC 3.4.24.-
• EC 3.4.24.82

Background

ADAMTS18 (A disintegrin and metalloproteinase with thrombospondin motifs 18) is a 1,221 amino acid protein that is thought to function as a tumor suppressor. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. ADAMTS18 is downregulated in carcinoma cell lines by methylation of its promoter. Forced expression of ADAMTS18 in carcinoma cells can lead to significant inhibition of cell growth, suggesting that it plays a significant role as a tumor suppressor. Mutations in ADAMTS18 are the cause of Knobloch syndrome type 2 (KNO2) and ADAMTS18 has also been linked to carotid artery occlusion, vitreoretinal degeneration, retinal detachment, myopia, cataracts, nasopharyngitis, pancreatic cancer, colorectal cancer, cerebritis breast cancer, melanoma and pancreatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for ADAMTS18 ELISA Kit (NBP2-66442)(1)

Publication using NBP2-66442

• Cláudia G, Rosauro V, Miquel DF et al. Endothelial transcriptomic analysis identifies biomarkers of severe and cerebral malaria JCI Insight. 2023-11-22 [PMID: 37788095] (ELISA, Human)

FAQs for ADAMTS18 ELISA Kit (NBP2-66442). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ADAMTS18