Human ADAMTS18 ELISA Kit (Colorimetric)

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Human ADAMTS18 ELISA Kit (Colorimetric) [NBP2-66442] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human ADAMTS18 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human ADAMTS18 in samples. No significant cross-reactivity or interference between Human ADAMTS18 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.77%
Intra-Assay
CV% < 4.7%
Spike Recovery
84-111%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ADAMTS18

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human ADAMTS18 ELISA Kit (Colorimetric)

  • A disintegrin and metalloproteinase with thrombospondin motifs 18
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 18
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 21
  • ADAM metallopeptidase with thrombospondin type 1 motif, 18
  • ADAM-TS 18
  • ADAM-TS18
  • ADAMTS-18
  • ADAMTS21
  • disintegrin and metalloprotease-like protein
  • EC 3.4.24.-
  • EC 3.4.24.82

Background

ADAMTS18 (A disintegrin and metalloproteinase with thrombospondin motifs 18) is a 1,221 amino acid protein that is thought to function as a tumor suppressor. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. ADAMTS18 is downregulated in carcinoma cell lines by methylation of its promoter. Forced expression of ADAMTS18 in carcinoma cells can lead to significant inhibition of cell growth, suggesting that it plays a significant role as a tumor suppressor. Mutations in ADAMTS18 are the cause of Knobloch syndrome type 2 (KNO2) and ADAMTS18 has also been linked to carotid artery occlusion, vitreoretinal degeneration, retinal detachment, myopia, cataracts, nasopharyngitis, pancreatic cancer, colorectal cancer, cerebritis breast cancer, melanoma and pancreatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ADAMTS18