ADAM28 Overexpression Lysate (Native)

Images

 
Western Blot: ADAM28 Overexpression Lysate (Adult Normal) [NBL1-07310] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADAM28.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ADAM28 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC217775 and based on accession number NM_014265. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens ADAM metallopeptidase domain 28 (ADAM28), transcript variant 1, mRNA.
Gene
ADAM28

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ADAM28

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ADAM28 Overexpression Lysate (Native)

  • a disintegrin and metalloproteinase domain 28
  • ADAM metallopeptidase domain 28
  • ADAM23MDC-L
  • ADAM28
  • disintegrin and metalloproteinase domain-containing protein 28
  • Dtgn1
  • EC 3.4.24
  • EC 3.4.24.-
  • eMDC II
  • eMDCII
  • Epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich proteinII
  • MDCL
  • MDCLADAM 28
  • MDC-Lm
  • MDC-Ls
  • Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L
  • metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L
  • Protein-L

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. Alternative splicing results in two transcript variants. The shorter version encodes a secreted isoform, while the longer version encodes a transmembrane isoform. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ADAM28 Lysate (NBL1-07310) (0)

There are no publications for ADAM28 Lysate (NBL1-07310).
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Video Protocols

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Bioinformatics

Gene Symbol ADAM28
Entrez