ADAM28 Antibody (2248B) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ELISA
• Clone: 2248B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

ADAM28 Antibody (2248B) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human ADAM28 protein
  Leu72 & Leu191-Ala623, with a C-terminal 6-His tag
  Accession # Q9UKQ2
• Specificity: Detects human ADAM28 in direct ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM28 Antibody (2248B) [Alexa Fluor® 532]

• a disintegrin and metalloproteinase domain 28
• ADAM metallopeptidase domain 28
• ADAM23MDC-L
• ADAM28
• disintegrin and metalloproteinase domain-containing protein 28
• Dtgn1
• EC 3.4.24
• EC 3.4.24.-
• eMDC II
• eMDCII
• Epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich proteinII
• MDCL
• MDCLADAM 28
• MDC-Lm
• MDC-Ls
• Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L
• metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L
• Protein-L

Background

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3).  ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9).  R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.