Acylglycerol Kinase Antibody - BSA Free

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 10% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Acylglycerol Kinase Antibody [NBP1-32163] - Methanol-fixed Hep3B, using Acylglycerol kinase (NBP1-32163) antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Acylglycerol kinase antibody detects Acylglycerol kinase protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. Acylglycerol kinase antibody diluted at 1:500.

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - 50 ug mouse heart lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Acylglycerol kinase antibody detects Acylglycerol kinase protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Acylglycerol kinase antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Acylglycerol kinase antibody detects Acylglycerol kinase protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Acylglycerol kinase antibody diluted at 1:500.

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] -Mouse tissue extract (30ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Acylglycerol Kinase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Acylglycerol Kinase Antibody - BSA Free (NBP1-32163) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Acylglycerol Kinase. The exact sequence is proprietary.
• Localization: Mitochondrion membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Acylglycerol Kinase Antibody - BSA Free

• acylglycerol kinase
• AGK
• EC 2.7.1.107
• EC 2.7.1.94
• FLJ10842
• FLJ10842
• hAGK
• HsMuLK
• mitochondrial
• MULK
• MULKhsMuLK
• Multi-substrate lipid kinase

Background

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AGK
• Entrez:
  • 55750(Human)
• Uniprot:
  • Q53H12(Human)