ACTR3 Antibody (JB33-44)

Western Blot: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution.Positive control:Lane 1: A431 Lane 2: Mouse placentaLane 3: Mouse thymus

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (JB33-44) [NBP2-75395] - Staining Arp3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of paraffin-embedded rat kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin.

Flow Cytometry: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of HL-60 cells with Arp3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (JB33-44) [NBP2-75395] - Staining Arp3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (JB33-44) [NBP2-75395] - Staining Arp3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of paraffin-embedded human placenta tissue using anti-Arp3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Arp3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of paraffin-embedded mouse kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin.

Product Discontinued

ACTR3 Antibody (JB33-44) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human ACTR3 aa 308-418 / 418. (SwissProt: P61158 Human; SwissProt: Q99JY9 Mouse; SwissProt: Q4V7C7 Rat)
• Localization: Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTR3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ACTR3 Antibody (JB33-44)

• actin-related protein 3
• ACTR3
• ARP3 (actin-related protein 3, yeast) homolog
• ARP3 actin-related protein 3 homolog (yeast)
• ARP3
• ARP3Actin-like protein 3

Background

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTR3