Actin Alpha 1 Cardiac Muscle Antibody (959606) [Unconjugated]

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alpha -Cardiac Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat alpha -Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 1.7 µg/mL for 1 ...read more
alpha -Cardiac Actin was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/Rat alpha -Cardiac Actin Monoclonal Antibody ...read more
Western blot shows lysates of human, mouse, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat alpha -Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) followed by ...read more
Simple Western lane view shows lysates of human, mouse, and rat heart tissue, loaded at 0.2 mg/mL. A specific band was detected for alpha -Cardiac Actin at approximately 50 kDa (as indicated) using 2 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
959606
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

Actin Alpha 1 Cardiac Muscle Antibody (959606) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human alpha -Cardiac Actin
Asp3-Phe377
Accession # P68032
Specificity
Detects human alpha -Cardiac Actin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Immunocytochemistry 5-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (959606) [Unconjugated]

  • ACTC
  • ACTC1
  • actin, alpha, cardiac muscle 1
  • alpha-Cardiac Actin
  • ASD5
  • Cardiac alpha-Actin
  • CMD1R
  • CMH11
  • LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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