ACOT11 Antibody

Western Blot: ACOT11 Antibody [NBP2-15246] - Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ACOT11 Antibody [NBP2-15246] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACOT11 Antibody Summary

• Description: Novus Biologicals Rabbit ACOT11 Antibody (NBP2-15246) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACOT11. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACOT11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (86%), Rat (86%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ACOT11 Antibody

• Acyl-CoA thioester hydrolase 11
• acyl-CoA thioesterase 11BFIT1
• Adipose-associated thioesterase
• BFIT2
• BFITDKFZp667O1916
• Brown fat-inducible thioesterase
• EC 3.1.2.-
• EC 3.1.2.1
• KIAA0707acyl-coenzyme A thioesterase 11
• STARD14
• START domain containing 14
• THEAStAR-related lipid transfer (START) domain containing 14
• THEM1
• thioesterase superfamily member 1
• thioesterase, adipose associated

Background

This gene encodes a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of a similar murine protein in brown adipose tissue is induced by cold exposure and repressed by warmth. Expression of the mouse protein has been associated with obesity, with higher expression found in obesity-resistant mice compared with obesity-prone mice. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACOT11
• Uniprot:
  • Q8WXI4(Human)