ABI1 Overexpression Lysate


Western Blot: ABI1 Overexpression Lysate (Adult Normal) [NBL1-07204] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABI1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

ABI1 Overexpression Lysate Summary

Transient overexpression lysate of abl-interactor 1 (ABI1), transcript variant 4 The lysate was created in HEK293T cells, using Plasmid ID RC201264 and based on accession number NM_001012752. The protein contains a C-MYC/DDK Tag.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
51.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ABI1 Overexpression Lysate

  • Abelson interactor 1
  • ABI1
  • ABI-1
  • abl interactor 1
  • Abl-binding protein 4
  • ABLBP4
  • abl-interactor 1
  • Abl-interactor protein 1 long
  • E3B1
  • eps8 binding protein
  • Eps8 SH3 domain-binding protein
  • Eps8-binding protein
  • interactor protein AblBP4
  • nap1 binding protein
  • Nap1-binding protein
  • NAP1BP
  • spectrin SH3 domain binding protein 1
  • Spectrin SH3 domain-binding protein 1
  • SSH3BP
  • SSH3BP1


The protein encoded by this gene has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling. Several transcript variants encoding multiple isoforms have been found for this gene. Transcript Variant: This variant (4) lacks two alternate in-frame coding exons compared to variant 1. The resulting isoform (d) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol ABI1