ABCB8 Antibody

Product Discontinued

ABCB8 Antibody Summary

• Immunogen: Peptide with sequence C-KKPEGPRSHQHK corresponding to C-Terminus according to NP_009119.2.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCB8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave an approx. 22 kDa band in lysates of HeLa and Jurkat after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1 kDa band according to NP_009119.2. The 22 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ABCB8 Antibody

• ATP-binding cassette, sub-family B (MDR/TAP), member 8
• EC 3.6.3
• EST328128
• M-ABC1mitochondrial ABC protein
• Mitochondrial ATP-binding cassette 1
• mitochondrial

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, it may involve the compartmentalization and transport of heme, as well as peptides, from the mitochondria to the nucleus and cytosol. This protein may also play a role in the transport of phospholipids into mitochondrial membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCB8 Antibody (NBP1-20869)(1)

Publication using NBP1-20869

• Hogue DL, Liu L, Ling V. Identification and characterization of a mammalian mitochondrial ATP-binding cassette membrane protein. J Mol Biol 1999-01-08 [PMID: 9878413]

Bioinformatics

• Gene Symbol: ABCB8
• Entrez:
  • 11194(Human)
• Uniprot:
  • NP_009119.2(Human)