ABCB5 Antibody

Images

 
Western Blot: ABCB5 Antibody [NBP1-50547] - A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified antibody.
Immunohistochemistry-Paraffin: ABCB5 Antibody [NBP1-50547] - Analysis of anti-ABCB5 antibody with human breast at concentration 10 ug/ml.
Western Blot: ABCB5 Antibody [NBP1-50547] - K562 cell line lysates (35ug/lane).

Product Details

Summary
Product Discontinued
View other related ABCB5 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50547
    • Availability
      Product Discontinued

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ABCB5 Antibody Summary

Immunogen
KLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human ABCB5.
Clonality
Polyclonal
Host
Rabbit
Gene
ABCB5
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50-1:100
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 10 ug/ml
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-50547 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for ABCB5 Antibody

  • ABCB5 P-gp
  • ABCB5
  • ABCB5alpha
  • ABCB5beta
  • ATP-binding cassette protein
  • ATP-binding cassette sub-family B member 5
  • ATP-binding cassette, sub-family B (MDR/TAP), member 5
  • EC 3.6.3
  • EC 3.6.3.44
  • EST422562
  • P-glycoprotein ABCB5

Background

ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCB5 Antibody (NBP1-50547)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ABCB5 Antibody (NBP1-50547). (Showing 1 - 1 of 1 FAQs).

  1. I am writing in regards to your product 'ABCB5 Antibody (NBP1-50547)'. I am interested in performing FACS analysis with it. I would like to ask you, however, whether the epitope recognized by the antibody is located extracellularly. Or does the antibody recognize an epitope intracellularly and thus for FACS analysis cells should be first permeabilized?
    • The immunogen used to generate NBP1-50547 is located on the N-terminal which for ABCB5 is the extracellular domain.

Secondary Antibodies

 

Isotype Controls

Additional ABCB5 Products

Bioinformatics Tool for ABCB5 Antibody (NBP1-50547)

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol ABCB5
Entrez
Uniprot