ABCA9 Antibody

Immunohistochemistry-Paraffin: ABCA9 Antibody [NB100-93469] - Staining of paraffin embedded Human Testis with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: ABCA9 Antibody [NB100-93469] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABCA9 Antibody Summary

• Description: Novus Biologicals Goat ABCA9 Antibody (NB100-93469) is a polyclonal antibody validated for use in IHC, ELISA and Flow. Anti-ABCA9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QRVVQELEMENIQD corresponding to internal region according to NP_525022.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCA9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Approx. 170 kDa band observed in human brain (cerebellum) lysates (calculated MW of 184 kDa band according to NP_525022.2).
• Theoretical MW: 184 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA9 Antibody

• ATP-binding cassette A9
• ATP-binding cassette sub-family A member 9
• ATP-binding cassette, sub-family A (ABC1), member 9
• DKFZp686F2450
• EC 3.6.3
• EC 3.6.3.41
• EST640918
• MGC75415

Background

This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This gene is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of this gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA9 Antibody (NB100-93469)(1)

Publication using NB100-93469

• Piehler A, Kaminski WE, Wenzel JJ et al. Molecular structure of a novel cholesterol-responsive A subclass ABC transporter, ABCA9 Biochem Biophys Res Commun 2002-07-12 [PMID: 12150964]

Bioinformatics

• Gene Symbol: ABCA9
• Entrez:
  • 10350(Human)
• Uniprot:
  • Q8IUA7(Human)