AAK1 Antibody (702425)

Western blot shows lysates of IMR-32 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AAK1 Monoclonal Antibody (Catalog # MAB6886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for AAK1 (Long Form) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 702425
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AAK1 Antibody (702425) Summary

• Immunogen: E. coli-derived recombinant human AAK1
  Ser704-Ile822
  Accession # Q2M2I8
• Specificity: Detects human AAK1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AAK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AAK1 Antibody (702425)

• AAK1
• Adaptor-associated kinase 1
• AP2 associated kinase 1
• AP2-associated protein kinase 1
• DKFZp686F03202
• DKFZp686K16132
• EC 2.7.11.1
• FLJ23712
• FLJ25931
• FLJ31060
• FLJ45252
• KIAA1048FLJ42882
• MGC138170
• MGC164568
• MGC164570

Background

AP2-associated protein kinase 1 (AAK1) is a 100 kDa intracellular kinase that binds and phosphorylates NUMB and the m2 subunit of AP2. NUMB and AP2 are adaptor proteins which are involved in the assembly and sorting of clathrin coated pits during receptor-mediated endocytosis. AAK1 contains one catalytic domain (aa 46‑315) and a C-terminal clathrin-binding region. Alternate splicing of human AAK1 generates a 145 kDa long isoform that contains an additional clathrin-binding domain. Within aa 704‑822, human AAK1 shares 95% aa sequence identity with mouse and rat AAK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AAK1 Antibody (MAB6886)(1)

Publication using MAB6886

• M Le Duff, J Gouju, B Jonchère, J Guillon, B Toutain, A Boissard, C Henry, C Guette, E Lelièvre, O Coqueret Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy Cell Death Dis, 2018-02-07;9(2):199. 2018-02-07 [PMID: 29415991] (Western Blot, Human)

Bioinformatics

• Gene Symbol: AAK1
• Uniprot:
  • Q2M2I8()