AAK1 Antibody (702425) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 702425
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

AAK1 Antibody (702425) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human AAK1
  Ser704-Ile822
  Accession # Q2M2I8
• Specificity: Detects human AAK1 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AAK1 Antibody (702425) [Alexa Fluor® 532]

• AAK1
• Adaptor-associated kinase 1
• AP2 associated kinase 1
• AP2-associated protein kinase 1
• DKFZp686F03202
• DKFZp686K16132
• EC 2.7.11.1
• FLJ23712
• FLJ25931
• FLJ31060
• FLJ45252
• KIAA1048FLJ42882
• MGC138170
• MGC164568
• MGC164570

Background

AP2-associated protein kinase 1 (AAK1) is a 100 kDa intracellular kinase that binds and phosphorylates NUMB and the m2 subunit of AP2. NUMB and AP2 are adaptor proteins which are involved in the assembly and sorting of clathrin coated pits during receptor-mediated endocytosis. AAK1 contains one catalytic domain (aa 46‑315) and a C-terminal clathrin-binding region. Alternate splicing of human AAK1 generates a 145 kDa long isoform that contains an additional clathrin-binding domain. Within aa 704‑822, human AAK1 shares 95% aa sequence identity with mouse and rat AAK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.