Human A33/GPA33 ELISA Kit (Colorimetric)

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ELISA: Human A3 3/GPA33 ELISA Kit (Colorimetric) [NBP3-30758] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue Homogenates, Cell Lysates and other Biological Fluids
Standard Curve Range
0.625 - 40 ng/mL (example only; lot dependent)
Sensitivity
0.238 ng/mL (example only; lot dependent)

Order Details

Human A33/GPA33 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.625 - 40 ng/mL (example only; lot dependent)
Sensitivity
0.238 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GPA33

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human A33/GPA33 ELISA Kit (Colorimetric)

  • A33
  • cell surface A33 antigen
  • glycoprotein A33 (transmembrane)
  • Glycoprotein A33
  • GPA33
  • MGC129986
  • MGC129987
  • transmembrane glycoprotein A33

Background

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GPA33