A-RAF 293T Cell Transient Overexpression Lysate

• Reactivity: Hu
• Applications: WB

A-RAF 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-ARAF antibody by Western Blots. Plasmid: pCMV-ARAF full-length
• Specificity: A-RAF 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: ARAF

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for A-RAF 293T Cell Transient Overexpression Lysate

• ARaf
• A-Raf
• ARAF1A-RAF
• EC 2.7.11
• EC 2.7.11.1
• Oncogene ARAF1
• PKS
• PKS2
• PKS2A-Raf proto-oncogene serine/threonine-protein kinase
• Proto-oncogene A-Raf
• Proto-oncogene A-Raf-1
• Proto-oncogene Pks
• RAFA1
• Ras-binding protein DA-Raf
• serine/threonine-protein kinase A-Raf
• v-raf murine sarcoma 3611 viral oncogene homolog 1
• v-raf murine sarcoma 3611 viral oncogene homolog

Background

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ARAF