A-RAF Antibody

Western Blot: A-RAF Antibody [H00000369-D01P] - Analysis of ARAF expression in human placenta.

Immunocytochemistry/Immunofluorescence: A-RAF Antibody [H00000369-D01P] - Analysis of antibody to ARAF on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: A-RAF Antibody [H00000369-D01P] - Analysis of ARAF expression in transfected 293T cell line by ARAF polyclonal antibody.Lane 1: ARAF transfected lysate(67.60 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: A-RAF Antibody [H00000369-D01P] - Analysis of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with anti-ARAF rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

A-RAF Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ARAF (NP_001645.1, 1 a.a. - 606 a.a.) full-length human protein. MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
• Specificity: ARAF - v-raf murine sarcoma 3611 viral oncogene homolog,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARAF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for A-RAF Antibody

• ARaf
• A-Raf
• ARAF1A-RAF
• EC 2.7.11
• EC 2.7.11.1
• Oncogene ARAF1
• PKS
• PKS2
• PKS2A-Raf proto-oncogene serine/threonine-protein kinase
• Proto-oncogene A-Raf
• Proto-oncogene A-Raf-1
• Proto-oncogene Pks
• RAFA1
• Ras-binding protein DA-Raf
• serine/threonine-protein kinase A-Raf
• v-raf murine sarcoma 3611 viral oncogene homolog 1
• v-raf murine sarcoma 3611 viral oncogene homolog

Background

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARAF
• Entrez:
  • 369(Human)
• Uniprot:
  • NP_001645.1(Human)