5-HT2B Antibody (1014838) [Unconjugated]

5-HT2B was detected in immersion fixed paraffin-embedded sections of mouse cerebellum using Mouse Anti-Human 5-HT2B Monoclonal Antibody (Catalog # MAB10322) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Purkinje neurons.

• Reactivity: Hu
• Applications: IHC
• Clone: 1014838
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

5-HT2B Antibody (1014838) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human 5- HTR2B
  Accession # P41595
• Specificity: Detects human 5-HTR2B in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5-HT2B Antibody (1014838) [Unconjugated]

• 5-HT(2B)
• 5HT2B
• 5-HT2B
• 5-HT-2B
• 5-HT2B5-HT 2B receptor
• 5-hydroxytryptamine (serotonin) receptor 2B
• 5-hydroxytryptamine 2B receptor
• 5-hydroxytryptamine receptor 2B
• HTR2B
• Serotonin receptor 2B

Background

HTR2B is a G-protein coupled receptor for serotonin. The 5HT2 receptors mediate many of the central and peripheral physiologic functions of serotonin. 5HT2B plays a role in the regulation of dopamine and serotonin release, serotonin uptake and in the regulation of extracellular dopamine and serotonin levels. It also plays a role in pulmonary vasoconstriction and regulating cardiac structure and functions. 5HT2B receptors were shown to be expressed in human failing heart and have been strongly implicated in drug induced valvular heart disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT2B Antibody (MAB10322)(1)

Publication using MAB10322

• Li, ZY;Mao, YQ;Hua, Q;Sun, YH;Wang, HY;Ye, XG;Hu, JX;Wang, YJ;Jiang, M; Serotonin receptor 2B induces visceral hyperalgesia in rat model and patients with diarrhea-predominant irritable bowel syndrome World journal of gastroenterology 2024-03-14 [PMID: 38596485] (Immunohistochemistry, Human, Rat)

Bioinformatics

• Uniprot:
  • P41595()