5-HT1A Overexpression Lysate

Images

 
Western Blot: 5HT1A Receptor Overexpression Lysate (Adult Normal) [NBP2-10679] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 5HT1A Receptor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

5-HT1A Overexpression Lysate Summary

Description
Transient overexpression lysate of 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A) The lysate was created in HEK293T cells, using Plasmid ID RC218011 and based on accession number NM_000524. The protein contains a C-MYC/DDK Tag.
Gene
HTR1A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
45.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for 5-HT1A Overexpression Lysate

  • 5-HT1a receptor
  • 5HT1A
  • 5-HT1A
  • 5-HT-1A
  • 5-HT1A5-hydroxytryptamine receptor 1A
  • 5-hydroxytryptamine (serotonin) receptor 1A
  • ADRB2RL1
  • ADRB2RL15HT1a
  • ADRBRL1
  • G protein coupled receptor
  • G-21
  • guanine nucleotide-binding regulatory protein-coupled receptor
  • HTR1A
  • PFMCD
  • Serotonin receptor 1A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-26091
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-56350
Species: Hu, Mu, Rt
Applications: WB
MAB5764
Species: Hu
Applications: CyTOF-ready, Flow, IHC
NBP2-99205
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-00178
Species: Hu, Mu, Sh
Applications: Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA, WB
NBP3-05546
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, WB
AF1445
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP1-88191
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NLS590
Species: Hu, Pm
Applications: IHC, IHC-P
NB100-56352
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, WB
NBP1-78403
Species: Hu
Applications: ICC/IF, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB300-731
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
NBP1-89396
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-10679
Species: Hu
Applications: WB

Publications for 5-HT1A Lysate (NBP2-10679) (0)

There are no publications for 5-HT1A Lysate (NBP2-10679).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 5-HT1A Lysate (NBP2-10679) (0)

There are no reviews for 5-HT1A Lysate (NBP2-10679). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 5-HT1A Lysate (NBP2-10679) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-10679

Additional 5-HT1A Products

Bioinformatics Tool for 5-HT1A Lysate (NBP2-10679)

Discover related pathways, diseases and genes to 5-HT1A Lysate (NBP2-10679). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 5-HT1A Lysate (NBP2-10679)

Discover more about diseases related to 5-HT1A Lysate (NBP2-10679).
 

Pathways for 5-HT1A Lysate (NBP2-10679)

View related products by pathway.

PTMs for 5-HT1A Lysate (NBP2-10679)

Learn more about PTMs related to 5-HT1A Lysate (NBP2-10679).
 

Research Areas for 5-HT1A Lysate (NBP2-10679)

Find related products by research area.

Blogs on 5-HT1A

There are no specific blogs for 5-HT1A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our 5-HT1A Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol HTR1A