5-HT1A Antibody

Product Discontinued

5-HT1A Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 291-340 of Human SR-1A.
• Specificity: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29375322).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of SR-1A (F321) pAb in extracts from HepG2 cells.
  Immunohistochemistry (IHC) analyzes of SR-1A (F321) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-HT1A Antibody

• 5-HT1a receptor
• 5HT1A
• 5-HT1A
• 5-HT-1A
• 5-HT1A5-hydroxytryptamine receptor 1A
• 5-hydroxytryptamine (serotonin) receptor 1A
• ADRB2RL1
• ADRB2RL15HT1a
• ADRBRL1
• G protein coupled receptor
• G-21
• guanine nucleotide-binding regulatory protein-coupled receptor
• HTR1A
• PFMCD
• Serotonin receptor 1A

Background

The members of the G protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. beta-adrenergic bound to adrenaline activates adenylyl cyclase, while alpha2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the alpha2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories, designated SR-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT1A Antibody (NB100-92418)(1)

Publication using NB100-92418

• Perrier JF, Rasmussen HB, Jorgensen LK, Berg RW. Intense Activity of the Raphe Spinal Pathway Depresses Motor Activity via a Serotonin Dependent Mechanism Front Neural Circuits 2017-01-09 [PMID: 29375322] (IHC-Fr, Mouse)

FAQs for 5-HT1A Antibody (NB100-92418). (Showing 1 - 1 of 1 FAQ).

1. Would you please be so kind as to inform us of the immunogen characteristics used to develop the 5HT1A antibody (NB100-92418)?
   • The immunogen sequence of NB100-92418 is amino acids 301-350 of sequence P08908.

Bioinformatics

• Gene Symbol: HTR1A
• Entrez:
  • 3350(Human)
  • 15550(Mouse)
  • 24473(Rat)
• Uniprot:
  • P08908(Human)