4EBP1 Antibody [DyLight 405]

• Reactivity: Hu, Mu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: DyLight 405

4EBP1 Antibody [DyLight 405] Summary

• Immunogen: E. coli-derived recombinant human 4EBP1
  Ser2-Ile118
  Accession # Q13541
• Specificity: Detects human and mouse 4EBP1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EIF4EBP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for 4EBP1 Antibody [DyLight 405]

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4EBP1