Human 15-Lipoxygenase 2 ELISA Kit (Colorimetric)

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ELISA: Human 15-Lipoxygenase 2 ELISA Kit (Colorimetric) [NBP2-68193] - Samples were spiked with high concentrations of Human 15-Lipoxygenase 2 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human 15-Lipoxygenase 2 ELISA Kit (Colorimetric) [NBP2-68193] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human 15-Lipoxygenase 2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human ALOX15B in samples. No significant cross-reactivity or interference between Human ALOX15B and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.53%
Intra-Assay
CV% < 5.15%
Spike Recovery
86-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ALOX15B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human 15-Lipoxygenase 2 ELISA Kit (Colorimetric)

  • 15-lipoxygenase 2
  • 15-LOX-B
  • arachidonate 15-lipoxygenase 2
  • arachidonate 15-lipoxygenase B
  • Arachidonate 15-lipoxygenase type II
  • arachidonate 15-lipoxygenase, second type
  • arachidonate 15-lipoxygenase, type B
  • arachidonate omega(6) lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.33,15-LOX-215S-lipoxygenase

Background

15 Lipoxygenase 2 encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ALOX15B