15-Lipoxygenase 2 Antibody (4A7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 2 Antibody (4A7) [H00000247-M05] - Analysis of monoclonal antibody to ALOX15B on HeLa cell. Antibody concentration 10 ug/ml

ELISA: 15-Lipoxygenase 2 Antibody (4A7) [H00000247-M05] - Detection limit for recombinant GST tagged ALOX15B is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

15-Lipoxygenase 2 Antibody (4A7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALOX15B (AAH35217.1, 181 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQA
• Specificity: ALOX15B - arachidonate 15-lipoxygenase, type B (4A7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALOX15B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 15-Lipoxygenase 2 Antibody (4A7) - Azide and BSA Free

• 15-lipoxygenase 2
• 15-LOX-B
• arachidonate 15-lipoxygenase 2
• arachidonate 15-lipoxygenase B
• Arachidonate 15-lipoxygenase type II
• arachidonate 15-lipoxygenase, second type
• arachidonate 15-lipoxygenase, type B
• arachidonate omega(6) lipoxygenase
• EC 1.13.11
• EC 1.13.11.33,15-LOX-215S-lipoxygenase

Background

This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 15-Lipoxygenase 2 Antibody (H00000247-M05)(1)

Publication using H00000247-M05

• Valkonen, S;Holopainen, M;Colas, RA;Impola, U;Dalli, J;Kakela, R;Siljander, PR;Laitinen, S; Lipid mediators in platelet concentrate and extracellular vesicles: Molecular mechanisms from membrane glycerophospholipids to bioactive molecules Biochim Biophys Acta Mol Cell Biol Lipids 2019-04-10 [PMID: 30980920] (WB, Human)

Bioinformatics

• Gene Symbol: ALOX15B
• Entrez:
  • 247(Human)
• Uniprot:
  • AAH35217(Human)